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Table 3 Effect of different incubation periods, carbon and nitrogen sources on chitosanase production by Fusarium oxysporum D18

From: Production, statistical evaluation and characterization of chitosanase from Fusarium oxysporum D18

Variable

Dry weight (g)

Final pH

Protein content (mg /ml)

Chitosanase activity (U/ ml)

Incubation period (days)

 3

0.09

7

1.770

0.657

 5

0.16

7

2.144

0.941

 7

0.19

7

2.256

1.165

 9

0.14

6

2.230

1.072

 11

0.06

5.5

1.924

0.830

Carbon source

 Fructose

0.07

6.5

1.270

0.825

 Sucrose

0.10

6

2.396

1.127

 Dextrose

0.06

6

0.496

0.390

 Lactose

0.12

6.5

2.428

1.191

 Glucose

0.09

6

1.096

0.960

 Starch

0.06

6

0.640

0.365

 Chitosan

0.10

7

2.256

1.160

Nitrogen source

 Yeast extract

0.11

6

2.428

1.060

 KNO3

0.14

6

1.128

0.928

  (NH4)2SO4

0.25

6

2.23

1.133

 NH4Cl

0.07

4

2.574

1.220

 NaNO2

0.05

6

1.124

0.472