Table 5 Fractional precipitation of chitosanase using different saturations of ammonium sulphate
From: Production, statistical evaluation and characterization of chitosanase from Fusarium oxysporum D18
Precipitating agent | Concentration (%) | Final pH | Protein content (mg / ml) | Total protein content (mg/fraction) | Recovered protein (%) | Chitosanase activity (U / ml) | Specific activity (U/mg protein) | Total activity (U/fraction) | Recovered activity (%) |
|---|---|---|---|---|---|---|---|---|---|
Control | - | 6 | 2.715 | 543 | 100 | 1.990 | 0.733 | 398 | 100 |
Ammonium sulphate | 25 | 6 | 1.926 | 5.778 | 1.064 | 0.627 | 0.326 | 1.881 | 0.473 |
35 | 6 | 2.436 | 7.308 | 1.346 | 1.139 | 0.468 | 3.417 | 0.859 | |
50 | 6 | 3.586 | 10.758 | 1.981 | 1.910 | 0.533 | 5.73 | 1.440 | |
65 | 6 | 6.100 | 18.300 | 3.370 | 2.766 | 0.453 | 8.298 | 2.085 | |
75 | 6 | 7.826 | 23.478 | 4.324 | 3.436 | 0.439 | 10.308 | 2.590 | |
85 | 6 | 5.518 | 16.554 | 3.049 | 2.982 | 0.540 | 8.946 | 2.248 | |
Total | Â | Â | Â | 82.176 | 15.134 | Â | Â | 38.850 | 9.693 |