Molecular characterization of Carbapenem-resistant Escherichia coli isolates from sewage at Mulago National Referral Hospital, Kampala: a cross-sectional study

Table 1 Gene-specific primer pairs targeting the different Carbapenemase genes and their corresponding sizes in base pairs

Gene	Primer sequence	Size (bp)	Reference
bla _KPC	F 5’ ATG TCA CTG TAT CGC CGT CT 3’	498	(Mushi et al. 2014)
bla _KPC	R 5’ TTT TCA GAG CCT TAC TGC CC 3’	498	(Mushi et al. 2014)
bla _IMP-1	F 5’ TGA GCA AGT TAT CTG TAT TC 3’	232	(Mushi et al. 2014)
bla _IMP-1	R 5’ TTA GTT GCT TGG TTT TGA TG 3’	232	(Mushi et al. 2014)
bla _IMP-2	F 5’ GGC AGT CGC CCT AAA ACA AA 3’	232	(Mushi et al. 2014)
bla _IMP-2	R 5’ TAG TTA CTT GGC TGT GAT GG 3’	232	(Mushi et al. 2014)
bla _VIM	F 5’ GAT GGT GTT TGG TCG CAT A 3’	390	(Joji et al. 2019)
bla _VIM	R 5’ CGA ATG CGC AGC ACC AG 3’	390	(Joji et al. 2019)
bla _NDM	F 5’ GGT TTG GCG ATC TGG TTT TC 3’	621	(Zainol Abidin et al. 2015)
bla _NDM	R 5’ CGG AAT GGC TCA TCA CGA TC 3’	621	(Zainol Abidin et al. 2015)
bla _OXA-48	F 5’ TTG GTG GCA TCG ATT ATC GG 3’	238	(Mushi et al. 2014)
bla _OXA-48	R 5’ GAG CAC TTC TTT TGT GAT GGC 3’	238	(Mushi et al. 2014)

ISSN: 1869-2044