Molecular characterization of Carbapenem-resistant Escherichia coli isolates from sewage at Mulago National Referral Hospital, Kampala: a cross-sectional study

Table 2 Genes and their primer sequences for molecular characterization of E. coli adopted from (Dias et al. 2012)

Gene	Primer	Sequence (5’–3’)	Size of amplicon (Bp)	Reference
Eae	Sk1	CCCGAATTCGGCACAAGCATAAGC	881	(Madic et al. 2010)
Eae	Sk2	CCCGGATCCGTCTCGCCAGTATTCG	881	(Madic et al. 2010)
Stx	Vtcom-u	GAGCGAAATAATTTATATGTG	518	(Sato et al. 2003)
Stx	Vtcom-d	TGATGATGGCAATTCAGTAT	518	(Sato et al. 2003)
Est	EST-F	ATT TTT MTT TCT GTA TTR TCT T	190	(López-Saucedo et al. 2003)
Est	EST-R	CAC CCG GTA CAR GCA GGA TT	190	(López-Saucedo et al. 2003)
Elt	ELT-F	GGC GAC AGA TTA TAC CGT GC	450	(López-Saucedo et al. 2003)
Elt	ELT-R	CGG TCT CTA TAT TCC CTG TT	450	(López-Saucedo et al. 2003)
paH	IpaIII	GTTCCTTGACCGCCTTTCCGATACCGTC	619	(Sethabutr et al. 1994)
paH	IpaIV	GCCGGTCAGCCACCCTCTGAGAGTC	619	(Sethabutr et al. 1994)
aggR	aggRKs1	GTATACACAAAAGAAGGAAGC	254	(Shin et al. 2015)
aggR	aggRKas2	ACAGAATCGTCAGCATCAGC	254	(Shin et al. 2015)

ISSN: 1869-2044