Molecular characterization of Carbapenem-resistant Escherichia coli isolates from sewage at Mulago National Referral Hospital, Kampala: a cross-sectional study

Table 3 Primers for detection of PAI markers

Gene	Primer sequence (5’-3’)	Size of product (bp)	Reference
PAI I₅₃₆	F: TAA TGC CGG AGA TTC ATT GTC	1800	(Sabaté et al. 2006)
PAI I₅₃₆	R: AGG ATT TGT CTC AGG GCT TT	1800	(Sabaté et al. 2006)
PAI II₅₃₆	F: CAT GTC CAA AGC TCG AGC C	1000	(Sabaté et al. 2006)
PAI II₅₃₆	R: CTA CGT CAG CGT GGC TTT	1000	(Sabaté et al. 2006)
PAI IV₅₃₆	F: AAG GAT TCG CTG TTA CCG GAC	300	(Sabaté et al. 2006)
PAI IV₅₃₆	R: TCG TCG GGC AGC GTT TCT TCT	300	(Sabaté et al. 2006)
PAI I_J96	F: TCG TGC TCA GGT CCG GAA TTT	400	(Sabaté et al. 2006)
PAI I_J96	R: TGG CAT CCC ACA TTA TCG	400	(Sabaté et al. 2006)
PAI II_J96	F: GGA TCC ATG AAA ACA TGG TTA ATG GG	2300	(Sabaté et al. 2006)
PAI II_J96	R: GAT ATT TTT GTT GCC ATT GGT TAC C	2300	(Sabaté et al. 2006)
PAI I_CFT073	F: GGA CAT CCT GTT ACA GCG CGC	930	(Sabaté et al. 2006)
PAI I_CFT073	R: TCG CAA CAA ATC ACA GCG AAC	930	(Sabaté et al. 2006)
PAI II_CFT073	F: ATG GAT GTT GTA TCG CGC	400	(Sabaté et al. 2006)
PAI II_CFT073	R: ACG AGC ATG TGG ATC TGC	400	(Sabaté et al. 2006)

ISSN: 1869-2044