Table 3 Operation table of PAL enzyme activity determination
Reagent name | Volume in sample tube (μL) | Blank tube (μL) |
|---|---|---|
Sample | 20 | - |
Reagent 1 | 780 | 800 |
Reagent 2 | 200 | 200 |
Reagents were mixed well and placed in a 30 °C accurate water bath for 30 min | ||
 Reagent 3 | 40 | 40 |