Table 1 Detailed morphological, biochemical, and physiological characteristics of bacterial isolates obtained from Cholistan desert used in the present study
Characteristics | B. subtilis AH-08 | B. cereus AH-67 | B. badius SH-16 | P. koreensis SH-29 | Characteristics | B. subtilis AH-08 | B. cereus AH-67 | B. badius SH-16 | P. koreensis SH-29 |
|---|---|---|---|---|---|---|---|---|---|
Isolation from/ medium | Soil/ L. B | Soil/ L. B | Soil/L. B | Soil/L. B | KOH test | - | - | - | - |
Colony color / edges/elevation | Off-white/ undulate/flat elevation | Cottony White/entire/flat | Pale-Yellow/ filamentous/flat | White/lobate/flat | Catalase |  +  |  +  |  +  |  +  |
Colony morphology/size | Gummy, Wrinkled/ medium | Filamentous/ large | Round/ Large | Wrinkled/ medium | Biofilm | Strong | Intermediate | intermediate | Week |
Cell shape/motility | Rod/ highly motile | Rod/ highly motile | Rod/ motile | Rod/ motile | Temperature tolerance | 50 °C | 50 °C | 50 °C | 50 °C |
16S rRNA accession | MZ779051 | MZ779053 | ON340744 | MZ779057 | Salt tolerance | 7.5% | 2.5% | 7.5% | 7.5% |
Gram staining | Positive | Positive | Positive | Negative | Siderophore Production |  +  | - | - |  +  |
P-Solubilization (zone diameter mm) |  + (5) |  + (4) |  + (3) |  + (1.83) | Zinc Solubilization | - |  + (2.21) |  +  |  + (1.5) |
Ca- Solubilization (zone diameter mm) |  +  |  + (4.5) |  +  | - | IAA Production (ppm) |  +  + (50) |  +  + (100) |  +  + (75) |  +  |
Antibiotic Resistance | |||||||||
 Gentamycin (10 mg) | S | S | S | S | Streptomycin (10 mg) | R | R | R | R |
 Rifampicin (5 mg) | R | R | R | R | Ciprofloxacin (5 mg) | S | S | S | S |
 Nalidixic acid (30 mg) | MS | MS | MS | MS | Kanamycin (30 mg) | R | R | R | R |
 Ceftriaxone (30 mg) | S | S | S | S | Neomycin (10 mg) | S | S | S | S |
 Amikacin (30 mg) | R | R | R | R |  |  |  |  |  |
Carbon source utilization and enzymatic reaction | |||||||||
 ONPG |  +  |  +  |  +  |  +  | SODIUM CITRATE |  +  | - | - |  +  |
 SODIUM MALONATE | - | - | - |  +  | LYSINE DECORBOXYLASE |  +  |  +  |  +  |  +  |
 ARGININE DIHYDROLASE | - | - | - | - | ORNITHINE DECORBOXYLASE | - | - | - | - |
 H2S PRODUCTION | - | - | - | - | UREA HYDROLYSIS |  +  | - |  +  |  +  |
 TRYPTOPHANE DEAMINASE | - | - | - |  +  | INDOLE | - | - | - | - |
 ACETION |  +  |  +  |  +  |  +  | GELATIN HYDROLYSIS |  +  |  +  |  +  |  +  |
 ACID FROM GLUCOSE |  +  |  +  |  +  |  +  | NO3/N2 PRODUCTION |  +  |  +  |  +  | -/ +  |
 ACID FROM MALTOSE |  +  |  +  |  +  |  +  | ACID FROM SUCROSE |  +  |  +  |  +  |  +  |
 ACID FROM MANNITOL |  +  | - |  +  |  +  | ACID FROM ARABINOSE |  +  |  +  |  +  |  +  |
 ACID FROM RHAMNOSE |  +  |  +  |  +  |  +  | ACID FROM SORBITOL |  +  | - |  +  |  +  |
 ACID FROM INOSITOL | - | - |  +  |  +  | ACID FROM ADONITOL | - | - | - | - |
 ACID FROM MELIBIOSE |  +  |  +  | - |  +  | ACID FROM RAFFINOSE | - | - |  +  | - |