Table 2 Sequence of primer pairs and their respective product size designed in this study for qPCR analysis of heat induced genes in maize
Gene | Primers Sequence (5` to 3`) | Product Size (bp) |
|---|---|---|
HSP1 | Forward: ATTGCGACCACACCTCACAA | 190 |
Reverse: GAAGATGTACCAGGGCGAGG | ||
HSP18 | Forward:CGATCCGACATCCGAGAGAT | 340 |
Reverse:GGTACTTGGCGTCGTCCTC | ||
HSP70 | Forward: CAACGACACACGACAAGCAG | 284 |
Reverse: TTTGCTAGAGCTTGCCCACA | ||
HSP101 | Forward: CTGAGACGGGGATGAAGTCG | 224 |
Reverse: TCACGGGCTTATCTACACGC | ||
Actin | Forward: ATGGCTGACGGTGAGGACATCCAGCC | 205 |
| Â | Reverse: AGGTGAGGATACCCCTCTTGGATTGG | Â |