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Fig. 8 | Annals of Microbiology

Fig. 8

From: Process analysis of the anaerobe Phocaeicola vulgatus in a shake flasks and fermenter reveals pH and product inhibition

Fig. 8

Comparison of P. vulgatus cultivations in shake flask in duplicates and benchtop bioreactor. Offline samples OD600nm (red squares), pH (green circles), and glucose (pink diamonds) of shake flask (filled symbols) and bioreactor cultivations (open symbols). Medium = DMMG, cGlucose = 6 g L−1, cbuffer = 50 mM MOPS, T = 37 °C, nfermenter = 600 rpm, nshake flask = 100 rpm, VL,fermenter = 1500 mL, VL,shake flask = 50 mL, vvm = 0.2 min−1, gas mix = 1% CO2 in 99% N2. After 6 h, 0.5 mL antifoam was added to the fermenter

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