Bioethanol production from sugarcane molasses by co-fermentation of Saccharomyces cerevisiae isolate TA2 and Wickerhamomyces anomalus isolate HCJ2F-19

Table 5 Complete design matrix in terms of predicted and actual responses using Response surface-central composite design (CCD)

Run	Mixing rate A	Temperature B	Molasses concentration C	Incubation time D	Bioethanol concentration (g.L⁻¹)		Bioethanol yield (%)
Run	Mixing rate A	Temperature B	Molasses concentration C	Incubation time D	Actual	Predicted	Actual	Predicted
1	105	35	35	36	17.58	16.17	33.81	31.09
2	105	25	35	72	16.92	15.64	32.53	30.08
3	155	25	25	36	17.00	17.56	32.69	33.77
4	130	30	30	54	29.38	28.95	56.49	55.67
5	80	30	30	54	13.00	12.91	25.00	24.82
6	130	30	20	54	22.00	21.11	42.31	40.61
7	130	30	30	54	28.77	28.95	55.32	55.67
8	130	20	30	54	21.00	19.89	40.39	38.26
9	105	35	25	72	17.00	17.78	32.69	34.19
10	155	25	35	72	19.00	20.51	36.54	39.44
11	180	30	30	54	22.75	22.11	43.75	42.53
12	155	35	25	72	21.00	20.23	40.39	38.91
13	130	30	30	90	12.00	11.06	23.08	21.27
14	105	35	25	36	17.00	16.70	32.69	32.11
15	155	25	25	72	19.00	19.93	36.54	38.34
16	155	35	35	72	18.00	17.91	34.62	34.44
17	105	25	25	72	15.00	15.88	28.85	30.55
18	130	30	30	54	28.00	28.95	53.85	55.67
19	105	25	25	36	12.00	11.61	23.08	22.34
20	105	25	35	36	12.00	13.98	23.08	26.89
21	155	25	35	36	22.00	20.74	42.31	39.89
22	130	30	30	54	28.66	28.95	55.11	55.67
23	130	30	40	54	21.00	21.16	40.39	40.69
24	130	40	30	54	22.00	22.38	42.31	43.04
25	130	30	30	54	28.89	28.95	55.56	55.67
26	155	35	35	36	21.00	21.33	40.39	41.02
27	130	30	30	54	30.00	28.95	57.69	55.67
28	130	30	30	18	10.00	10.21	19.23	19.64
29	155	35	25	36	20.25	21.05	38.94	40.47
30	105	35	35	72	14.00	14.65	26.92	28.16

ISSN: 1869-2044