Table 6 Analysis of variance (ANOVA) for bioethanol production of co-fermenting S. cerevisiae TA2 and W. anomalus HCJ2F-19 using CCD experiments

Model

3455.78

14

246.84

45.94

 < 0.0001

Significant

A-Mixing rate

470.58

1

470.58

87.59

 < 0.0001

B-Temperature

34.24

1

34.24

6.37

0.0233

C-Molasses

0.0099

1

0.0099

0.0018

0.9663

D-Incubation time

4.00

1

4.00

0.7437

0.4021

AB

9.47

1

9.47

1.76

0.2042

AC

2.46

1

2.46

0.4577

0.5090

AD

13.27

1

13.27

2.47

0.1369

BC

31.00

1

31.00

5.77

0.0297

BD

37.54

1

37.54

6.99

0.0184

CD

25.12

1

25.12

4.68

0.0472

A²

829.50

1

829.50

154.39

 < 0.0001

B²

386.86

1

386.86

72.00

 < 0.0001

C²

386.86

1

386.86

72.00

 < 0.0001

D²

2126.14

1

2126.14

395.73

 < 0.0001

Residual

80.59

15

5.37

Lack of Fit

72.08

10

7.21

4.23

0.0624

Not significant

Pure Error

8.51

5

1.70

Total

3536.38

29