Fig. 5
From: Screening and identification of an Enterobacter ludwigii strain expressing an active β-xylosidase
Agarose electrophoretogram of DNA and SDS-PAGE electrophoretogram of proteins. a Lane M: DL 5000 DNA Marker. Lane 1: PCR product using by primers LY-3Nde1 and LY-4Xho1. Lane 2: PCR product using by primers LY-5Nco1 and LY-6Xho1. b Lane M: premixed protein marker (low) (TaKaRa). Lane 1: extracellular supernatant of BL21(DE3) containing empty pET-28a(+). Lane 2: extracellular supernatant of BL21(DE3) containing His-Xyl62. Lane 3: purified His-Xyl62. Lane 4: extracellular supernatant of BL21(DE3) containing Xyl62-His. Lane 5: purified Xyl62-His