Fig. 5

The 1.5% ethidium bromide-stained agarose gel showing PCR amplification from the selected native B. thuringiensis stains using cry1Ab gene primer combinations. Legends: M—Molecular Weight Marker (100 bp, Thermo Scientific, USA), Lane1—BNG_Bta1, Lane2—SHVM_Bta2, Lane3—MYS_Bta3, Lane4—VJP_Bta4, Lane5—no Amplification, Lane6—CMR_Bta6, Lane7—no amplification, Lane8—BID_Bta8, Lane 9—AN_Bta1, Line10—AN_Bta2, Lane11–13—no amplification, Lane 14—genomic negative control (without genomic DNA), Lane15—water control (Water served as a template instead of DNA).