Table 1 Primers used to screen Aphidicidal cry genes from native B. thuringiensis isolates
Primer | Sequences | Positions | Gene(s) recognized | Product size (bp) | GenBank accession no. | Reference |
|---|---|---|---|---|---|---|
Set IF | 5′-TATGCWCAAGCWGCCAATYTWCATYT-3′ | 3 domain blocks 1 and 5 | cry1, cry2, cry3, cry4, cry7, and almost all cry genes | - | - | Noguera and Ibarra 2010 |
Set IR | 5′-GGRATAAATTCAATTYKRTCWA-3′ |  | ||||
Set IIF | 5′-TTTAGATATTGTTGCAWTATKKYC-3′ | 3 domain block 2 and 5 | cry5, cry8, cry10, cry12,cry13, cry14, cry20, cry40 and cry44 | |||
Set IIR | 5′-GGRATAAATTCAATTYKRTCWA-3′ | |||||
Set IIIF | 5′-TATGCWCAAGCWGCCAATYTWCATYT-3′ | 3 domain blocks 1 and 4 | cry16 and cry18 | |||
Set IIIR | 5′-CATAACGTAGWYTTAYCTKAWT-3′ | |||||
UNcry1(+) | 5′-TRACRHTDDBDGTATTAGAT-3′ | 726 | cryI | 1500–1600 | - | Seifinejad et al. 2008 |
UNcry1(-) | 5′ -MDATYTCTAKRTCTTGACTA-3′ | 2268 | ||||
Spcry1Aa(+) | 5′-TTCCCTTTATTTGGGAATGC-3′ | 1023 | cry1Aa | 1286 | M11250 | |
UNcry1(-) | 5′-MDATYTCTAKRTCTTGACTA-3′ | 2268 | ||||
SPcry1Ab(+) | 5′- CGGATGCTCATAGAGGAGAA-3′ | 940 | cry1Ab | 1371 | M13898 | |
UNcry1(-) | 5′-MDATYTCTAKRTCTTGACTA-3′ | 2268 | ||||
UNcry2(+) | 5′-GTTATTCTTAATGCAGATGAATGGG-3′ | 1444 | cry2Ab | 546 | M23724 | |
SPcry2Ab(-) | 5′-TGGCGTTAACAATGGGGGGAGAAAT-3′ | 1965 | ||||
SPcry3A(+) | 5′-TGGTGCCCCGTCTAAACTGAGTGT-3′ | 180 | cry3A | 951 | U10985 | Swamy et al. 2013 (reference Walters and English 1995 and Jouzani et al. 2017) |
UNcry3(–) | 5′-CATCTGTTGTTTCTGGAGGCAAT-3′ | 1131 | ||||
SPcry4A(+) | ACAGAATCAGCAGTATGGTGG | 156–176 | cry4A | 443 | Y00423 | Present study (reference Porcar et al. 2009) |
SPcry4A(-) | CGCAATCACTAGGATTAGGAGG | 577–598 |