Fig. 5

Schematic representation of the DNA fragments deleted in S. Typhi ΔF4, S. Typhi ΔF44, or S. Typhi ΔF73 strains that were less resistant to different pH values. Genes probably involved in invasion and replication of macrophages are indicated in bold. S. Typhi ∆F4 lack two putative IS element transposases (STY0114, STY0115), one hypothetical protein (STY0117), an operon involved in l-arabinose metabolism (araDAB-araC or STY0118-STY0121), a DedA-family integral membrane protein (yabI or STY0122), the thiamine operon (a thiamine transporter ATP-binding subunit, thiamine ABC transporter membrane component and thiamine-binding periplasmic protein precursor, thiQP-tbpA, or STY0123-STY0124-STY0125), a transcriptional regulator sgrR (STY0127), the enzymes responsible for the biosynthesis of leucine from valine (leuABCD or STY0129 to STY0132), the leu operon leader peptide (leuL or STY0133), the leucine transcriptional activator LeuO (leuO or STY0134), the acetolactate synthase III large and small subunits (ilvI and ilvH or STY0135 and STY0136), and the gene that encodes a fructose repressor (fruR or STY0138). S. Typhi ∆F44, this fragment lacks genes such as a hypothetical protein (STY1496), the osmC osmotically inducible protein C (osmC or STY1497), HlyE hemolysin (hylE or STY1498), a hypothetical protein (STY1499), two putative secreted protein (STY1501 and STY1502), putative glycogen debranching protein (glgx or STY1505), the putative aminotransferase (STY1507), a hypothetical protein (STY1509), putative isomerase (STY1513), putative regulatory protein (STY1514), putative multidrug efflux protein (STY1517), hypothetical protein (STY1518), membrane transport protein (STY1519), putative alcohol dehydrogenase (STY1520), putative regulatory protein (STY1521), putative secreted hydrolase (STY1522), the hydrogenases (hyaACDEF or STY1523-STY1530), putative ATP/GTP-binding protein (STY1531), hypothetical proteins (STY1533-STY1535), putative aldehyde-dehydrogenase (STY1536), putative regulatory protein (STY1537), sugar efflux transporter (STY1538), hypothetical protein (STY1539), the multiple antibiotic resistance protein marR, marA, and marB (STY1540-STY1542), two hypothetical proteins (STY1543-STY1544), putative periplasmic (STY1545), a competence damage-inducible protein A (STY1547), hypothetical protein (STY1548), dipeptidyl carboxypeptidase II (dcp or STY1549), putative oxydoreductase (STY1550), putative regulatory protein (STY1551), hypothetical protein (STY1552), putative membrane transport protein (STYSTY1554), starvation-sensing protein (rspAB or STY1555-STY1556), hypothetical protein (STY1558), putative secreted protein (STY1560), and a spermidine N1-acetyltransferase (speG or STY1561). S. Typhi ∆F73, genetic elements deleted in this fragment are hypothetical proteins (STY0286-STY0328), safAEBCD fimbrial assembly (STY0332-STY0337), sinR transcriptional regulator (STY0341), hypothetical protein (STY0342), tcfABCD fimbrial assembly (STY0345-STY0348), tinR transcriptional regulator (STY0349), hypothetical protein (STY0350), possible outer membrane adhesion (STY0351), probable secreted protein (STY0352), possible hydrolase (STY0353), possible acyl-CoA dehydrogenase (fadE or STY0354), phosphopentose isomerase (STY0355), and hypothetical proteins (STY0356-STY0357)